Preclinical Acute Toxicity Assessment of a Crude Polysaccharide Isolated from the Stem of Solanum Nigrum: A Preliminary Analysis
| Autor: | Faizan Naeem Razali, Nur Syahirah Izzati Rani, Muhammad Imran Kamil Mazian, Ahmad Naeem Mohd Nafi, Siti Hajar Musa |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Biomedical and Pharmacology Journal. 14:2131-2139 |
| ISSN: | 2456-2610 0974-6242 |
| Popis: | The polysaccharide isolated from Solanum nigrum was proven to possess an immunomodulatory effect and able to suppress the progression of tumor cells by proxy. However, data on the toxicity profile is still limited. The present preclinical study was conducted to investigate the toxicity potential of the crude polysaccharide sample. The acute toxicity experimental design was adapted from OECD 423 guideline. Nine female BALB/c mice were randomly divided into 3 groups, 3 mice per group (n=3). Mice in group A (first-step treatment) were orally administered with a single treatment of crude polysaccharide sample at concentration 2,000 mg/kg/bw (300 µL). Mice in group B (second-step treatment) were received the single treatment after 24 hours, depending on the observation of mice in group A. Mice in group C served as control. Mortality and clinical signs associated with toxicity were observed within 24 hours of treatment session and for the subsequence 14 days for delay-death detection. Mice body weight was recorded starting at day-0 until day-14 prior to sacrificing at day-15. Blood, liver, and kidney were harvested for toxicology assessment. Within 24 hours of treatment, 1 mouse in group A was found to died, while no mortality and delay-death were observed in groups B and C. Referring to OECD 423, it was estimated that the LD50 of the treated sample was 2,500–5,000 mg/kg/bw. No significant changes (p |
| Databáze: | OpenAIRE |
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