Enhancement of cadmium resistance by overexpression of BrMT3 in Arabidopsis
| Autor: | Young-Ock Ahn, Sang-Soo Kwak, Sun-Ha Kim, Won-Yong Song, Haeng-Soon Lee, Kwan-Sam Choi |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Cadmium
biology medicine.diagnostic_test fungi RuBisCO food and beverages chemistry.chemical_element Plant Science biology.organism_classification Green fluorescent protein Cell biology Chloroplast chemistry Western blot Arabidopsis Botany biology.protein medicine Metallothionein Agronomy and Crop Science Gene Biotechnology |
| Zdroj: | Journal of Plant Biotechnology. 36:68-74 |
| ISSN: | 1229-2818 |
| DOI: | 10.5010/jpb.2009.36.1.068 |
| Popis: | We have previously demonstrated that overexpression and characterization of Brassica rapa type-l metallothionein gene (BrMT1) in Arabidopsis which showed enhanced resistance to cadmium and ROS. Here, we present the consistent study of our previous report about BrMTs. BrMT3 expressing DTY167 cells showed resistance to Zn and Pb as well as Cd. Thus, we have developed the BrMT3 overexpression Arabidopsis to enhance capacity for metal stresses. Successful expression and localization were achieved using the rubisco transit peptides of RbcS-BrMT3-GFP protein, which was confirmed by western blot analysis with the GFP antibody and green fluorescence signal from the chloroplast. BrMT3 overexpression Arabidopsis plants exhibited a higher resistance to cadmium compared to control plants. This result indicates that BrMT3 would be applicable to the development of plants with enhanced resistance against heavy metal stresses. |
| Databáze: | OpenAIRE |
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