| Autor: |
Suzanne F. C. Miller-Delaney, Sudipto Das, David C. Henshall, Raymond L. Stallings |
| Rok vydání: |
2013 |
| Předmět: |
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| Zdroj: |
Methods in Molecular Biology ISBN: 9781627036061 |
| DOI: |
10.1007/978-1-62703-607-8_7 |
| Popis: |
Epigenetic modification of DNA by methylation of the cytosine present in CG dinucleotides constitutes a key regulatory mechanism in the control of gene expression in neurological diseases. In this chapter, we describe an in-depth methodology of methylated DNA immunoprecipitation used in combination with tiling microarrays (MeDIP-chip) in order to analyze genome-wide gene promoter methylation in the hippocampus of mice following status epilepticus (prolonged seizure). While a specific mouse model and array format are described, the method can be applied to DNA from many tissues to analyze the methylation status of promoter regions across whole genomes, using a wide range of available array formats (both custom designed and commercially catalogued). We conclude the chapter with the description of bisulfite sequencing validation of MeDIP-chip results. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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