| Autor: | Brian Cutting, Mauro Zurini, Francis Bitsch, Gabriele Fendrich, André Strauss, Patrick Graff, Janis Liebetanz, Wolfgang Jahnke |
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| Rok vydání: | 2003 |
| Předmět: | |
| Zdroj: | Journal of Biomolecular NMR. 26:367-372 |
| ISSN: | 0925-2738 |
| DOI: | 10.1023/a:1024013111478 |
| Popis: | Culture conditions for successful amino–acid-type selective isotope labeling of proteins expressed in Baculovirus-infected insect cells are described. The method was applied to the selective labeling of the catalytic domain of c-Abl kinase with 15N-phenylalanine, 15N-glycine, 15N-tyrosine or 15N-valine. For the essential amino acids phenylalanine, tyrosine and valine high 15N-label incorporation rates of ≥90% and approximately the expected number of resonances in the HSQC spectra were observed, which was not the case for the non-essential amino acid glycine. The method should be applicable to amino-acid-type selective isotope labeling of other recombinant proteins which have not been amenable to NMR analysis. |
| Databáze: | OpenAIRE |
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