Pretreatment with ablative fractional laser changes kinetics and biodistribution of topical 5-aminolevulinic acid (ALA) and methyl aminolevulinate (MAL)
| Autor: | Apostolos G. Doukas, Joshua Tam, Merete Haedersdal, Fernanda H. Sakamoto, William A. Farinelli, R. Rox Anderson |
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| Rok vydání: | 2014 |
| Předmět: |
Biodistribution
Protoporphyrin IX medicine.medical_treatment Photodynamic therapy Dermatology Hair follicle Fluorescence Porphyrin Molecular biology chemistry.chemical_compound medicine.anatomical_structure Methyl aminolevulinate Biochemistry chemistry parasitic diseases Fluorescence microscope medicine lipids (amino acids peptides and proteins) Surgery medicine.drug |
| Zdroj: | Lasers in Surgery and Medicine. 46:462-469 |
| ISSN: | 0196-8092 |
| DOI: | 10.1002/lsm.22259 |
| Popis: | Background and Objectives 5-Aminolevulinic acid (ALA) and methyl aminolevulinate (MAL) are porphyrin precursors used topically for photodynamic therapy (PDT). Previous studies have established that ablative fractional laser (AFXL) increases topical drug uptake. We evaluated kinetics and biodistribution of ALA- and MAL-induced porphyrins on intact and disrupted skin due to AFXL. Materials and Methods Two Yorkshire swine were exposed to CO2 AFXL (10.6 µm, 1,850 µm ablation depth) and subsequent topical application of ALA and MAL cream formulations (20%, weight/weight). Porphyrin fluorescence was quantified by digital fluorescence photography (30, 90, and 180 minutes) and fluorescence microscopy at specific skin depths (180 minutes). Results Porphyrins gradually formed over time, differently on intact and AFXL-disrupted skin. On intact skin (no AFXL), fluorescence photography showed that MAL initially induced higher fluorescence than ALA (t = 30 minutes MAL 21.1 vs. ALA 7.7 au, t = 90 minutes MAL 39.0 vs. ALA 26.6 (P |
| Databáze: | OpenAIRE |
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