De novo transcriptome sequencing of pakchoi (Brassica rapa L. chinensis) reveals the key genes related to the response of heat stress
| Autor: | Xu Hai, Chen Longzheng, Fan Xiaoxue, Yuan Xihan, Jinfeng Chen, Song Bo |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Molecular breeding Genetics Physiology Plant physiology RNA-Seq Plant Science Biology 01 natural sciences Heat stress Transcriptome 03 medical and health sciences 030104 developmental biology Brassica rapa KEGG Agronomy and Crop Science Gene 010606 plant biology & botany |
| Zdroj: | Acta Physiologiae Plantarum. 38 |
| ISSN: | 1861-1664 0137-5881 |
| DOI: | 10.1007/s11738-016-2269-5 |
| Popis: | Pakchoi is an important vegetable grown in worldwide that prefers a cool climate, and its growth is severely affected by high temperatures in the summer. Little is known for the molecular regulatory mechanisms of high-temperature stress in pakchoi. The limited availability of gene sequence has greatly affected molecular breeding and functional genomic analysis. In this study, we conducted comprehensive analyses for heat treatment in pakchoi by RNA-Seq. Finally, 64.29 million clean reads containing 32,666 unigenes with an N50 length of 1405 bp, and a total length of 33.39 Mb were identified. Overall, 11,024 SSRs located in 8404 unigenes were obtained; these findings will be very useful for molecular assisted breeding. Totally, 1220 differentially expressed genes (DEGs) were detected between the high-temperature group (TH) and the control (CK), among which 699 DEGs were up-regulated and 521 were down-regulated. The enrichment analysis indicated that 12 GO subcategories and 9 KEGG pathways were enriched significantly (P value |
| Databáze: | OpenAIRE |
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