Flow cytometric identification of Brassica napus protoplast fusion products
| Autor: | K. P. Pauls, P. V. Chuong |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
biology
fungi Brassica food and beverages Plant Science biochemical phenomena metabolism and nutrition Protoplast equipment and supplies biology.organism_classification Fluorescence Hypocotyl chemistry.chemical_compound Somatic fusion chemistry Chlorophyll Botany Fluorescein isothiocyanate Chlorophyll fluorescence |
| Zdroj: | Canadian Journal of Botany. 65:834-838 |
| ISSN: | 0008-4026 |
| DOI: | 10.1139/b87-113 |
| Popis: | Department of Crop Science, UniversiQ of Guelph, Guelph, Ont., Canada NIG 2WI Received July 1 1, 1986 PAULS, K. P., and CHUONG, P. V. 1987. Flow cytometric identification of Brassica napus protoplast fusion products. Can. J. Bot. 65: 834-838. A flow cytometric method to identify Brassica somatic hybrids has been developed. The procedure is based on the use of fluorescein isothiocyanate stained hypocotyl protoplasts and unstained mesophyll protoplasts as partners for polyethylene gly- col induced protoplast fusion. The fluorescein isothiocyanate stained hypocotyl protoplasts and unstained mesophyll proto- plasts were passed through the flow cytometer - cell sorter separately to maximize the sensitivity of the red and green detectors to chlorophyll and fluorescein isothiocyanate fluorescence, respectively, and to ensure that there was no spillover of chlorophyll fluorescence into the green detector or fluorescein isothiocyanate fluorescence into the red detector. When adjusted properly, suspensions of a single protoplast type gave populations that fell on either the green or red axis of a'two-dimensional green versus red fluorescence plot. With the same machine settings three populations of protoplasts could be identified in fusion mixtures of the two types of protoplasts: namely, the parental protoplasts that fell on the green or red axis as well as a third that had significant green and red fluorescence. PAULS, K. P., et CHUONG, P. V. 1987. Flow cytometric identification of Brassica napus protoplast fusion products. Can. J. Bot. 65 |
| Databáze: | OpenAIRE |
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