Acetylsalicylic Acid and Sodium Salicylate Inhibit LPS-lnduced NF-kB/c-Rel Nuclear Translocation, and Synthesis of Tissue Factor (TF) and Tumor Necrosis Factor alfa (TNF-a) in Human Monocytes

Autor:	Reidun Øvstebø, Peter Kierulf, K. B. Foss, Åse-Brit Westvik, L. T. N. Osnes, C. Okkenhaug, Gun-Britt Joø
Rok vydání:	1996
Předmět:	Lipopolysaccharide Monocyte Prostaglandin Hematology Molecular biology chemistry.chemical_compound Tissue factor medicine.anatomical_structure chemistry Biochemistry medicine Electrophoretic mobility shift assay Tumor necrosis factor alpha REL Sodium salicylate
Zdroj:	Thrombosis and Haemostasis. 76:0970-0976
ISSN:	2567-689X 0340-6245
DOI:	10.1055/s-0038-1650694
Popis:	SummaryWe have investigated the effects of acetylsalicylic acid and sodium salicylate on the LPS-induced synthesis of the pro-coagulant protein tissue factor (TF) and the pro-inflammatory protein tumor necrosis fac-tor-a (TNF-a), as well as the prostaglandin PGE2 in human monocytes. Both drugs dose-dependently inhibited LPS-induced TF and TNF-a synthesis at the mRNA and the protein level, and reduced PGE2 production. As evidenced by electro mobility shift assay (EMSA) and the use of a NF-kB prototypic probe, these drugs probably exert their inhibitory effects by interference with the nuclear translocation of NF-KB/c-Rel proteins. These data may expand the understanding of the anti-thrombotic and anti-inflammatory effects of these drugs when activation of monocytes occurs.
Databáze:	OpenAIRE
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