Location of a structural gene for xylose-H+ symport at 91 min on the linkage map of Escherichia coli K12

Autor: Peter J. F. Henderson, E O Davis, M C Jones-Mortimer
Rok vydání: 1984
Předmět:
Zdroj: Journal of Biological Chemistry. 259:1520-1525
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)43439-9
Popis: Mutations in the xylose-H+ transport activity of Escherichia coli K12 were isolated using Mud(ApRlac). The initial selection was for simultaneous acquisition of ampicillin and xylose resistance in an fda background. Colonies were then screened for xylose-inducible beta-galactosidase and for growth on xylose of their fda+ derivatives. Two of the xylose-positive derivatives were shown to be impaired in xylose-H+ symport in whole cells and in xylose transport into subcellular vesicles. Their xylose transport in whole cells showed increased sensitivity to arsenate. The site of prophage insertion was mapped to 91.4 min on the E. coli genome between pgi and malB. It is proposed that the gene for the xylose-H+ symport system be called xylE.
Databáze: OpenAIRE