Good performance of p16/ki-67 dual-stained cytology for surveillance of women treated for high-grade CIN
| Autor: | Renske D.M. Steenbergen, Daniëlle A.M. Heideman, Dorenda K E van Dijken, René H.M. Verheijen, Johan W M Spruijt, Theo J.M. Helmerhorst, Chris J.L.M. Meijer, Johannes Berkhof, Nicole J. Polman, Margot H. Uijterwaal, Folkert J. van Kemenade, Birgit I. Witte, W. Marchien van Baal, Peppino G.C.M. Graziosi, Ruediger Ridder, Peter J.F. Snijders |
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| Rok vydání: | 2016 |
| Předmět: |
Cancer Research
medicine.medical_specialty High-Grade CIN Cervical intraepithelial neoplasia Gastroenterology 03 medical and health sciences 0302 clinical medicine Internal medicine Cytology Biopsy medicine 030212 general & internal medicine Cervix Gynecology Cervical cancer Colposcopy biology medicine.diagnostic_test business.industry medicine.disease medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Ki-67 biology.protein business |
| Zdroj: | International Journal of Cancer. 140:423-430 |
| ISSN: | 0020-7136 |
| DOI: | 10.1002/ijc.30449 |
| Popis: | Women treated for high-grade cervical intraepithelial neoplasia (CIN) are at risk of recurrent CIN Grade 2 or worse (rCIN2+). Currently, posttreatment monitoring is performed using cytology or cytology/high-risk (hr)HPV cotesting. This study aimed to evaluate the performance of p16/Ki-67 dual-stained cytology (p16/Ki-67) for posttreatment monitoring. Three hundred and twenty-three women treated for high-grade CIN in the SIMONATH study underwent close surveillance by cytology, hrHPV and DNA methylation marker testing up to 12 months posttreatment. Histological endpoints were ascertained by colposcopy with biopsy at 6 and/or 12 months. p16/Ki-67 dual-staining was performed on residual liquid-based cytology samples obtained at, or shortly before biopsy collection. Clinical performance estimates of cytology, hrHPV, p16/Ki-67 testing and combinations thereof for the detection of rCIN2+ were determined and compared to each other. Sensitivity of p16/Ki-67 for rCIN2+ (69.2%) was nonsignificantly lower than that of cytology (82.1%; ratio 0.84, 95% CI: 0.71-1.01), but significantly lower than that of hrHPV testing (84.6%; ratio 0.82, 95% CI: 0.68-0.99). Specificity of p16/Ki-67 for rCIN2+ (90.4%) was significantly higher compared to both cytology (70.8%; ratio 1.28, 95% CI: 1.19-1.37) and hrHPV testing (76.2%; ratio 1.19, 95% CI: 1.12-1.26). Overall, hrHPV testing showed very high sensitivity, along with a good specificity. When considering cotesting, combined p16/Ki-67/hrHPV testing showed rCIN2+ sensitivity comparable to cytology/hrHPV cotesting (87.2% vs. 89.7%; ratio 0.97, 95% CI: 0.92-1.03), but with significantly increased specificity (74.2% vs. 58.1%; ratio 1.28, 95% CI: 1.19-1.38). Thus, when considered in combination with hrHPV, p16/Ki-67 might be an attractive approach for surveillance of women treated for high-grade CIN. |
| Databáze: | OpenAIRE |
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