Soft Substrate Culture to Mechanically Control Cardiac Myofibroblast Activation
| Autor: | Natalie M. Landry, Ian M.C. Dixon, Sunil G. Rattan |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cardiac fibrosis Cell Biology medicine.disease In vitro Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Cell culture In vivo medicine Primary cell Myofibroblast Immortalised cell line 030217 neurology & neurosurgery |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071613818 |
| Popis: | Two-dimensional cell culture is the primary method employed for proof-of-concept studies in most molecular biology labs. While immortalized cell lines are convenient and easy to maintain for extended periods in vitro, their inability to accurately represent genuine cell physiology-or pathophysiology-presents a challenge for drug discovery, as most results are not viable for the transition to clinical trial. The use of primary cells is a more biologically relevant approach to this issue; however, simulating in vitro what is observed in vivo is exigent at best. Primary cardiac fibroblasts are particularly difficult to maintain in a quiescent state, due to their innate phenotypic plasticity, and sensitivity to mechanical and biochemical stimulus. As conventional cell culture methods do not consider these factors, here we describe a method that limits environmental input (i.e., mechanical, nutritional, hormonal) to extend the physiological cardiac fibroblast phenotype in vitro. |
| Databáze: | OpenAIRE |
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