Gene expression of bovine endometrial epithelial cells cultured in matrigel
| Autor: | Nobuhiko Yamauchi, Daichi Nishino, Al Nur Md Iftekhar Rahman, Ken ichi Yamanaka, M.A.M. Yahia Khandoker, Ai Kotake, Mohamed E. El-Sharawy, Chi Sun Yun |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Matrigel Histology Stromal cell Chemistry Embryo Cell Biology Endometrium Molecular biology Molecular medicine Pathology and Forensic Medicine In vitro model 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Gene expression medicine Gene 030217 neurology & neurosurgery |
| Zdroj: | Cell and Tissue Research. 385:265-275 |
| ISSN: | 1432-0878 0302-766X |
| Popis: | Glandular epithelial cells (GE) in the endometrium are thought to support the elongation and survival of ruminant embryos by secreting histotrophs. In the present study, the gene expression of bovine endometrial epithelial cells cultured in matrigel was analyzed and examined whether it could be an in vitro model of GE. Bovine endometrial epithelial cells (BEE) and stromal cells (BES) were isolated from the slaughterhouse uteri and cultured in DMEM/F12 + 10% FBS. BEE showed the gland-like structure morphological changes when cultured in 15% matrigel but could not be identified in higher concentrations of the matrigel (30% or 60%). The expression of typical genes expressed in GE, SERPINA14 and GRP, was substantially high in matrigel-cultured BEE than in monolayer (P |
| Databáze: | OpenAIRE |
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