Increased Production of Bioactive Lysophosphatidic Acid by Serum Lysophospholipase D in Human Pregnancy1
| Autor: | Shuji Yamano, Minoru Irahara, Yumi Kanaya, Maki Miyake, Kenji Fukuzawa, Akira Tokumura |
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| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
Pregnancy Cell Biology General Medicine Biology medicine.disease Enzyme activator chemistry.chemical_compound medicine.anatomical_structure Endocrinology Reproductive Medicine chemistry Internal medicine Placenta Lysophosphatidic acid medicine Extracellular Alkylglycerophosphoethanolamine phosphodiesterase Gestation lipids (amino acids peptides and proteins) biological phenomena cell phenomena and immunity Intracellular |
| Zdroj: | Biology of Reproduction. 67:1386-1392 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod.102.004051 |
| Popis: | Lysophosphatidic acid (LPA) is a prototype of the lysophospholipid mediator family and has multiple effects in the female reproductive system. Although several metabolic routes have been reported for intracellular formation of LPA, a unique route involving lysophospholipase D, an extracellular enzyme that produces LPA in blood and body fluids, is particularly intriguing for its agonistic role. In this study, using an assay with radioactive palmitoyl-lysophosphatidylcholine, we found that lysophospholipase D activity producing palmitoyl-LPA in human serum gradually increased during pregnancy. Elevated activity of lysophospholipase D was not caused by changes in levels of their precursors, lysophosphatidylcholines, in nonpregnant women or in pregnant women at different gestational periods. With increasing length of gestation, the elevated activity in pregnant women was found to produce increasing proportions of LPA with a palmitoyl group versus other LPAs. These results suggest that LPA formed by increased activity of lysophospholipase D in blood might participate in maintenance of pregnancy. |
| Databáze: | OpenAIRE |
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