Low Pulmonary Expression of Epithelial Na+ Channel and Na+, K+-ATPase in Newborn Infants with Congenital Diaphragmatic Hernia
| Autor: | Anita Aperia, Björn Frenckner, Andreas Ringman Uggla, Marina Zelenina, Katarina von Schewelov |
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| Rok vydání: | 2010 |
| Předmět: |
Epithelial sodium channel
medicine.medical_specialty Pathology Lung Respiratory distress business.industry Congenital diaphragmatic hernia medicine.disease Pulmonary hypoplasia Endocrinology medicine.anatomical_structure Downregulation and upregulation Internal medicine Pediatrics Perinatology and Child Health medicine Animal studies Na+/K+-ATPase business Developmental Biology |
| Zdroj: | Neonatology. 99:14-22 |
| ISSN: | 1661-7819 1661-7800 |
| Popis: | Background: It has been suggested from several animal studies and clinical observations that congenital diaphragmatic hernia (CDH) with pulmonary hypoplasia is accompanied by a disturbed perinatal ion transport. This could lead to respiratory distress due to slower clearance of fetal lung fluid at birth. Objectives: The purpose of this study was to determine whether CDH is related to changes in the expression of three rate-limiting transporter proteins in lung epithelium at birth. Methods: Tracheal aspirate was collected from 12 newborn infants with CDH and from 8 newborn control patients. Sampling was performed at postnatal age 18 and at 43 h in the CDH group and at 18 h in the control group. The protein abundance of α-, β- and γ-epithelial Na+ channel (ENaC), aquaporin 5 and Na+, K+-ATPase α1 was analyzed using semiquantitative immunoblotting. Results: The levels of β-ENaC, γ-ENaC and Na+, K+-ATPase α1 collected at 18 h postnatally were significantly lower in CDH infants compared to control infants. In the CDH group, no significant difference in the expression of the ENaC subunits, Na+, K+-ATPase α1 or aquaporin 5 could be detected between the two sampling time points. Conclusions: This downregulation may result in an abnormal lung fluid absorption which could be an important mechanism behind the respiratory distress seen in newborn CDH patients. |
| Databáze: | OpenAIRE |
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