Galactosylated chitosan (GC)-graft-poly(vinyl pyrrolidone) (PVP) as hepatocyte-targeting DNA carrier

Autor:	Jong Eun Ihm, Yun-Jaie Choi, Inkyu Park, Won Jong Kim, Chong-Su Cho, Y.H. Park, Toshihiro Akaike, Su Il Kim
Rok vydání:	2003
Předmět:	Chemistry Ligand binding assay technology industry and agriculture Pharmaceutical Science macromolecular substances Conjugated system Dosage form Chitosan chemistry.chemical_compound Agarose gel electrophoresis Polymer chemistry Drug carrier Ethidium bromide DNA
Zdroj:	Journal of Controlled Release. 86:349-359
ISSN:	0168-3659
DOI:	10.1016/s0168-3659(02)00365-6
Popis:	Galactosylated chitosan was conjugated with poly(vinyl pyrrolidone) (PVP) as a hydrophilic group. The complex formation of GC-graft-PVP (GCPVP)/DNA complexes was confirmed by agarose gel electrophoresis. The morphology of the complex observed by atomic force microscopy had a compact and spherical shape, around 40 nm particle sizes at a charge ratio of 3. The binding strength of GCPVP 10K/DNA complex measured by ethidium bromide binding assay was superior to that of the GCPVP 50K/DNA one, probably attributable to its higher flexibility due to the smaller size, whereas the DNase I protection of GCPVP 10K/DNA complex was inferior to that of the GCPVP 50K/DNA one. This indicated that effective complex formation required both higher binding strength and minimal molecular weight of polycation enough to induce the condensation of DNA. The DNA-binding property of GCPVP mainly depended on the molecular weight of chitosan and composition of PVP.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::da4d32ec0c0fd38ed92f3b29dc864882 https://doi.org/10.1016/s0168-3659(02)00365-6 Zobrazit plný text záznamu Full Text from ScienceDirect