GIARDIA INTESTINALIS ASSEMBLAGES AMONG EGYPTIAN SYMPTOMATIC CHILDREN: PREVALENCE AND SEASONAL DISTRIBUTION IN CAIRO, EGYPT
| Autor: | Zein Abd Al Aal, Nesreen S. Saleh, Ayman A. El-Badry, Samir A. Taha |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Genetic diversity Veterinary medicine education.field_of_study Seasonal distribution biology Transmission (medicine) 030231 tropical medicine Population Giardia 030108 mycology & parasitology biology.organism_classification 03 medical and health sciences Diarrhea Giardia intestinalis 0302 clinical medicine medicine medicine.symptom education Nested polymerase chain reaction |
| Zdroj: | Journal of the Egyptian Society of Parasitology. 48:661-668 |
| ISSN: | 2090-2549 |
| DOI: | 10.21608/jesp.2018.76585 |
| Popis: | Giardia intestinalis is one the commonest enteric intestinal protozoan pathogens worldwide, notorious for its seasonal prevalence and wide age span of population infected G. intestinalis causes diarrhea in humans, especially in children. The present study aimed to evaluate the prevalence and genetic diversity of G. intestinalis isolated from Egyptian symptomatic children. A total of 176 faecal samples of children, from Aboul-Reesh Pediatrics Hospital, Cairo. Direct wet mount and formalin-ether sedimentation techniques were applied for microscopic determination of Giardia infection in faecal samples. DNA was extracted from positive stool samples and amplified by nested polymerase chain reaction (nPCR) using specific primers of G. intestinalis Trios Phosphate Isomerase (TPI) gene. Assemblages were determined using sequence analysis ofthe TPI gene .Out of the 176 samples, G. intestinalis was detected in 33 (18.75%) and 24 (13.63%) cases using Coproscopy and nPCR, respectively. There was a significant predominance of assemblage B (20 cases [83.33%]) versus assemblage A (4 cases [16.66%]) In this study, molecular analysis revealed that assemblages A and B are the most common types with a predominance of assemblage B. These findings suggest that anthroponotic transmission could bea dominant transmission route for giardiasis in Egypt. |
| Databáze: | OpenAIRE |
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