The insemination of goldfish (Carassium auratus) oocyte maturedin vitro

Autor: Zhang Shicui, Ma Yingjie, Shi Yingxian, Wang Renxue, Wu Xianhan, Wu Shangqin, Zhou Jing
Rok vydání: 1991
Předmět:
Zdroj: Chinese Journal of Oceanology and Limnology. 9:88-93
ISSN: 1993-5005
0254-4059
DOI: 10.1007/bf02849794
Popis: Full maturation of goldfish oocyte was inducedin vitro by 17 α-hydroxy-20β-dihydroprogesterone. The oocyte maturation involves GV migration to the periphery of the oocyte and germinal vesicle breakdown (GVBD). In the experiment, incubation duration for GVBD varied in different broods of oocytes. Generally, if the duration for GVBD was shorter than 6 h, oocytes would have a better chance to survive after maturation and insemination. The maturation of nucleus (GV) and cytoplasm are not synchronous. Cytoplasm maturation occurs several hs after GVBD. Oocytes inseminated 8–9 h after GVBD have the highest fertilizing and hatching rate. Fertilized ova maturedin vitro can develop to sexually mature adults capable of reproduction.
Databáze: OpenAIRE