ISOLATION AND CULTURE OF GRAPE PROTOPLASTS FROM EMBRYOGENIC SUSPENSION CULTURES AND LEAVES OF VITIS VINIFERA AND VITIS ROTUNDIFOLIA

Autor:	Y. Jittayasothom, X. Xu, J. Lu, D. Dalling, J. W. Grosser
Rok vydání:	2007
Předmět:	Plating efficiency Cell division Somatic cell Callus formation fungi food and beverages Horticulture Protoplast Biology Vitis rotundifolia Cell culture Botany wine wine.grape_variety Cultivar
Zdroj:	Acta Horticulturae. :787-790
ISSN:	2406-6168 0567-7572
DOI:	10.17660/actahortic.2007.738.105
Popis:	Somatic hybridization offers the possibility of manipulating plant genomes between species. Successful application of this technique in plant cultivar improvement requires a high efficient plant regeneration system from protoplasts. A method for preparing viable protoplasts from somatic embryogenic cell suspension cultures was established in Vitis vinifera grape 'Autumn Royal Seedless' and V. rotundifolia 'Tara'. Somatic hybridization was attempted between protoplast derived from embryogenic cell lines and leaf protoplasts isolated from in vitro-grown plants 'Orlando Seedless', 'Cabernet Sauvignon', and 'Alachua' by means of polyethylene glycol (PEG). When cultured on BH3 and modified MS basal media containing 20 g/L maltose and 0.5 g/L glutamine supplemented with 5.0 μM β-naphthoxyacetic acid (NOA), the fusion products exhibited cell wall formation, cell division, and callus formation about 4 days after the cultivation. The highest plating efficiency was obtained on MS basal medium.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::da22f95db108cd6549b3096499549cf2 https://doi.org/10.17660/actahortic.2007.738.105 Zobrazit plný text záznamu