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Purification Guide for the Isolation of Histidine-tagged Proteins with ChroDrip Columns by DALEX Biotech. ChroSpin-IMAC by DALEX Biotech offers a robust and convenient way to isolate polyhistidine-tagged protein from bacterial, mamalian, and insect cell cultures. Immobilized metal affinity chromatography (IMAC) is based on the interaction of the imidazole ring of histidine with transition metal ions immobilized on a solid support. Recombinant proteins with a 3 - 10 histidine fusion tag bind to these metal ions while unwanted proteins are removed by washing with excess binding buffer. Elution of the target protein is achieved by the addition of imidazole, EDTA or a low pH. Easy and quick small scale fusion protein purification from various sources. Low metal leaching from column. Tolerates reducing reagents (e.g. DTT up to 10 mM). The proprietary resin does not shrink or swell in aqueous buffers. High pressure stability. pH stability short term 2 - 8, long term 3 - 8. Excellent thermal stability up to 15 minutes at 120 °C in aqueous buffers at neutral pH. Can be dried for long term storage (80 °C for > 2 h). |
