| Autor: |
Wolfram Saenger, Beate Pfannemüller, Rolf Nuck, Claudia Niemann |
| Rok vydání: |
1990 |
| Předmět: |
|
| Zdroj: |
Carbohydrate Research. 197:187-196 |
| ISSN: |
0008-6215 |
| DOI: |
10.1016/0008-6215(90)84141-g |
| Popis: |
The preparation of low-molecular-weight amyloses with modified terminal groups in the range d.p. 10–20 was achieved by enzymic synthesis using phosphorylase from a potato crude extract. 4-Nitrophenyl α- d -maltooligoglycosides with a minimum chain length of five d -glucose units served as primers, providing good yields in a 10–12 fold molar excess of α- d -glucopyranosyl phosphate. Up to 89% conversion was obtained after 4 h at pH 5.8, 40°, and a phosphorylase activity of 30 units. The reaction products were analyzed by h.p.l.c. Elution profiles always revealed oligomers shorter than the primer. The preferred appearance of the maltotrioside after incubation of the primer with the enzyme crude extract indicated the presence of a disproportionation enzyme. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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