Mud crab Scylla paramamosain glutamate dehydrogenase: molecular cloning, tissue expression and response to hyposmotic stress

Autor: Yu Liu, Bing-Peng Xu, Guangxu Liu, You-Zhi Ma, Miao-An Shu, Xiao-Ling Guo, Jing-Ying Lu
Rok vydání: 2014
Předmět:
Zdroj: Fisheries Science. 81:175-186
ISSN: 1444-2906
0919-9268
DOI: 10.1007/s12562-014-0828-5
Popis: Glutamate dehydrogenase (GDH) is an important enzyme for the metabolism of glycine, proline and alanine, which are general osmolytes in aquatic animals. To explore the possible relationship between GDH expression and osmoregulation under hyposmotic stress, cDNA cloning of GDH and its expression, as well as free amino acid analysis in Scylla paramamosain, were investigated. GDH cDNA was first isolated from S. paramamosain and the 2269 bp sequence consisted of a 1622 bp open reading frame encoding 533 amino acids. GDH was predominantly expressed in muscle, which is a pool of amino acids. Under acute transfer from a salinity of 28 to 5 ppt, the haemolymph osmolality was significantly decreased at 6 h, and the expression of GDH in the muscle dropped sharply to 29.6% as compared to the control at 6 h (P
Databáze: OpenAIRE
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