Recent Advances in the Molecular Genetics of Paramecium1

Autor:	W. J. Haynes, Ching Kung, Yoshiro Saimi, Kit-Yin Ling, Roland Kissmehl
Rok vydání:	2000
Předmět:	Cloning Genetics medicine.medical_specialty biology Mutant Molecular cloning biology.organism_classification Microbiology Transformation (genetics) Molecular genetics medicine Gene silencing Paramecium Gene
Zdroj:	The Journal of Eukaryotic Microbiology. 47:11-14
ISSN:	1550-7408 1066-5234
DOI:	10.1111/j.1550-7408.2000.tb00003.x
Popis:	Paramecium continues to be used to study motility, behavior, exocytosis, and the relationship between the germ and the somatic nuclei. Recent progress in molecular genetics is described. Toward cloning genes that correspond to mutant phenotypes, a method combining complementation with microinjected DNA and library sorting has been used successfully in cloning several novel genes crucial in membrane excitation and in trichocyst discharge. Paramecium transformation en masse has now been shown by using electroporation or bioballistics. Gene silencing has also been discovered in Paramecium, recently. Some 200 Paramecium genes, full length or partial, have already been cloned largely by homology. Generalizing the use of gene silencing and related reverse-genetic techniques would allow us to correlate these genes with their function in vivo.
Databáze:	OpenAIRE
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