Melibiase in Immobilized Cells of Watermelon
| Autor: | M. Varadinova, V. Foltan, Karol Mičieta, M. Duricek, J. Stano, M. Fulmekova, E. Tokhtaeva |
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| Rok vydání: | 2005 |
| Předmět: |
Chromatography
biology Physical protection Melibiase activity Substrate (chemistry) Plant Science General Chemistry biology.organism_classification Suspension culture General Biochemistry Genetics and Molecular Biology Enzyme assay chemistry.chemical_compound Hydrolysis chemistry Biochemistry biology.protein Glutaraldehyde Citrullus |
| Zdroj: | Chemistry of Natural Compounds. 41:65-68 |
| ISSN: | 1573-8388 0009-3130 |
| Popis: | Cells of suspension culture Citrullus vulgaris cv. “Samara” were permeabilized by Tween 80 and immobilized by glutaraldehyde. The highest melibiase activity was at pH 5.4 and 60°C. The hydrolysis of substrate was linear for 3.5 h, reaching 65–70% conversion of the substrate. The cells, characterized by high enzyme activity and stability in long-term storage, showed convenient physico-mechanical properties (physical protection from shear forces and easy separation of product from biocatalysts). |
| Databáze: | OpenAIRE |
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