Chito-oligosaccharide production by chitinase of Streptococcus macrosporeus VTCC 940003 and their inhibition activities on Botrytis cinerea
| Autor: | D. V. Hop, Jacques Dommes, Vinh Van Hoang, Marie-France Versali, T. T. V. Anh, N. Q. Uyen |
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| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
fungi Polyacrylamide Plant Science Horticulture Biology Oligosaccharide biology.organism_classification Chitosan chemistry.chemical_compound chemistry Chitin Biochemistry Chitinase Spore germination biology.protein Agronomy and Crop Science Mycelium Botrytis cinerea |
| Zdroj: | European Journal of Plant Pathology. 161:185-193 |
| ISSN: | 1573-8469 0929-1873 |
| DOI: | 10.1007/s10658-021-02313-9 |
| Popis: | Detection of chitinase activity after electrophoresis in native polyacrylamide gels containing glycol chitin showed that Streptomyces macrosporeus VTCC 940003 produced several chitinases. Chito-oligosaccharides (COS) were produced from commercial chitosan using these chitinases. The degree of polymerization (DP) of the produced COS was confirmed by Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS). Moreover, the biological activities of the COS against Botrytis cinerea in vitro and in vivo were assessed. The COS preparation contained a mixture of oligomers with DP ranging from 2 to 10. The COS also showed inhibition activities on spore germination, mycelial growth and infection of B. cinerea on tomato leaves. |
| Databáze: | OpenAIRE |
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