Estimation of Total Phenolic Contents and In Vitro Antioxidant and Antimicrobial Activities of Various Solvent Extracts of Melissa officinalis L
Autor: | H. Mabrouki, D.E. Akretche, Catarina M.M. Duarte |
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Rok vydání: | 2017 |
Předmět: |
Multidisciplinary
Antioxidant Chemistry DPPH medicine.medical_treatment 010401 analytical chemistry food and beverages 04 agricultural and veterinary sciences Antimicrobial Ascorbic acid 040401 food science 01 natural sciences 0104 chemical sciences Solvent chemistry.chemical_compound 0404 agricultural biotechnology Polyphenol Officinalis medicine Food science Melissa officinalis |
Zdroj: | Arabian Journal for Science and Engineering. 43:3349-3357 |
ISSN: | 2191-4281 2193-567X |
DOI: | 10.1007/s13369-017-3000-6 |
Popis: | This study aimed to evaluate the antioxidant and antimicrobial activities of Melissa officinalis L. extracts. In order to estimate the best conditions to extract polyphenols from the plant studied, different solvents such as hexane, acetone, and ethanol were used. The polyphenols content was estimated by the Folin–Ciocalteu method. The antioxidant activity was assessed by different methods such as ORAC test and the scavenging of free radical DPPH, while ascorbic acid was used as a control. The antimicrobial activity of M. officinalis L. was assessed by the method of disk diffusion. The results obtained showed that ethanol was the most efficient solvent for extracting polyphenols from the M. officinalis L. The ethanol extract exhibited strong antioxidant and antimicrobial activities with an EC 50 of $$18.16 \pm 0.64\,\upmu \hbox {g}/ \hbox {mL}$$ and a strong inhibition against Staphylococcus aureus. The results obtained suggest that Algerian M. officinalis L. can be used as an important source of natural antioxidant and antimicrobial agent for food and pharmaceutical industries. |
Databáze: | OpenAIRE |
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