Human eosinophils express and release IL-13 following CD28-dependent activation

Autor: Nadine Roger, Amena Ben Younes, Monique Capron, Gaetane Woerly, Redwan Moqbel, Sylvie Loiseau, Paige Lacy
Rok vydání: 2002
Předmět:
Zdroj: Journal of Leukocyte Biology. 72:769-779
ISSN: 1938-3673
0741-5400
DOI: 10.1189/jlb.72.4.769
Popis: Human eosinophils produce a large number of cytokines, including immunoregulatory cytokines. Given that eosinophils store and release interleukin (IL)-4, a key cytokine in the pathogenesis of allergic inflammation, and that IL-4 and IL-13 share common biological functions, we investigated the possibility that IL-13 may be synthesized by these cells. Using flow cytometry and immunocytochemistry, we show that eosinophils synthesize and store IL-13. Granule localization was demonstrated after subcellular fractionation, and IL-13 immunoreactivity was localized to crystalloid, granule-enriched fractions. Furthermore, electron microscopic analyses specifically localized IL-13 to the dense cores of bicompartmental secondary granules. Upon CD28 ligation, IL-13 was released by eosinophils, whereas a combination of CD28 and immunoglobulin A complexes resulted in decreased IL-13 secretion. Furthermore, eosinophil-derived IL-13 exerts a biological effect, inducing CD23 expression on B cells. By having the capacity to synthesize and release IL-13, eosinophils may participate in the development and maintenance of the T helper cell type 2 response, a prominent feature of allergic diseases.
Databáze: OpenAIRE
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