High level synthesis of immunoglobulins in Chinese hamster ovary cells

Autor: C R Wood, A J Dorner, G E Morris, E M Alderman, D Wilson, R M O'Hara, R J Kaufman
Rok vydání: 1990
Předmět:
Zdroj: The Journal of Immunology. 145:3011-3016
ISSN: 1550-6606
0022-1767
DOI: 10.4049/jimmunol.145.9.3011
Popis: The expression of lambda L and microH chain cDNA was examined in Chinese hamster ovary cells. Each cDNA was linked to a different, amplifiable, selectable drug marker gene, and expression was monitored in the presence of increasing concentrations of the selective drugs. Cells were obtained that produced greater than 60 micrograms/10(6) cells/48 h of assembled antibody. This Chinese hamster ovary cell-synthesized IgM was polymeric, and exhibited specific hapten binding and C fixation. The expression strategy employed here may prove useful for the future production of genetically engineered antibodies and other multi-subunit proteins.
Databáze: OpenAIRE