High level synthesis of immunoglobulins in Chinese hamster ovary cells

Autor:	C R Wood, A J Dorner, G E Morris, E M Alderman, D Wilson, R M O'Hara, R J Kaufman
Rok vydání:	1990
Předmět:	Immunology Immunology and Allergy
Zdroj:	The Journal of Immunology. 145:3011-3016
ISSN:	1550-6606 0022-1767
DOI:	10.4049/jimmunol.145.9.3011
Popis:	The expression of lambda L and microH chain cDNA was examined in Chinese hamster ovary cells. Each cDNA was linked to a different, amplifiable, selectable drug marker gene, and expression was monitored in the presence of increasing concentrations of the selective drugs. Cells were obtained that produced greater than 60 micrograms/10(6) cells/48 h of assembled antibody. This Chinese hamster ovary cell-synthesized IgM was polymeric, and exhibited specific hapten binding and C fixation. The expression strategy employed here may prove useful for the future production of genetically engineered antibodies and other multi-subunit proteins.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d9946bc6f79e2af83c35d104ac572319 https://doi.org/10.4049/jimmunol.145.9.3011 Zobrazit plný text záznamu