SIRT1 gene polymorphism and its plasma concentration in men with no signs of cardiovascular pathology, residents of Podilia region of Ukraine
| Autor: | V. M. Zhebel, T. M. Kalinovskaya, A. O. Donets |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
business.industry General Medicine law.invention enzymes and coenzymes (carbohydrates) law Polymorphism (computer science) Internal medicine Blood plasma Genotype medicine Biomarker (medicine) Gene polymorphism business Genotyping SIRT1 Gene hormones hormone substitutes and hormone antagonists Polymerase chain reaction |
| Zdroj: | Reports of Vinnytsia National Medical University. 25:215-219 |
| ISSN: | 2522-9354 1817-7883 |
| DOI: | 10.31393/reports-vnmedical-2021-25(2)-06 |
| Popis: | Annotation. The aim of the work – on the basis of scientific literature to consider the current state of prediction and diagnosis of hypertension using biomarkers. A review analysis of literature sources of domestic and foreign authors on the polymorphism of the SIRT1 gene and the possibility of using data of the biomarker SIRT1 in the diagnosis and treatment of patients with cardiovascular pathology and the concentration of SIRT1 in the blood plasma of 79 practically healthy men, 40-60 years old, residents of the Podilia region of Ukraine with carriers of polymorphic SIRT1 genes, genotype GG, CC and CG was studied. The concentration of SIRT1 in the blood plasma of the examined men was determined by enzyme-linked immunosorbent assay. Genotyping of the SIRT1 gene was performed by polymerase chain reaction. Processing of the obtained results was performed using STATISTICA 6.0. Practically healthy men from the Podilia region of Ukraine are dominated by variants of the CC and CG genotypes. The concentration of SIRT1 in the blood plasma of men, residents of the Podilia region of Ukraine without signs of cardiovascular pathology is not associated with polymorphism of variants of the coding gene SIRT1. It is planned to use the obtained results for further study of SIRT1 concentration in carriers of polymorphic genes in men with stage I and II hypertension, which may have diagnostic value. |
| Databáze: | OpenAIRE |
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