Listeria induces NKDC to produce IFN-gamma via IL-12, IL-18 and TLR9 ligation (44.24)
| Autor: | George Plitas, Umer I. Chaudhry, T. Peter Kingham, Jennifer Stableford, Ronald P. DeMatteo |
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| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 178:S52-S52 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.178.supp.44.24 |
| Popis: | Introduction: Natural killer dendritic cells (NKDC, NK1.1+CD11c+) are innate effector cells that secrete IFN-γ when stimulated. Initial control of Listeria monocytogenes (LM) infection depends on IFN-γ. The mechanism of IFN-γ secretion by NKDC in response to LM is unknown. Methods: NKDC and NK (NK1.1+CD3−) cells were isolated by fluorescence activated cell sorting from the spleens of C57BL/6 mice and cultured with LM strain 10403s at a multiplicity of infection of 1 for 3 days. IL-18 (20ng/ml), IL-12 blocking antibody (10μg/ml), or an inhibitory CpG (iCpG) sequence (50μg/ml) was added. Supernatant IFN-γ was analyzed by cytometric bead array. Results: LM infection or IL-18 alone did not induce significant IFN-γ production by NK cells or NKDC, however the combination of IL-18 and LM induced substantial amounts of IFN-γ from NKDC (19.3±2.7 ng/ml), and to a lesser extent, NK cells (2.0±0.6 ng/ml). Addition of anti-IL-12 antibody abrogated the IL-18/LM induced IFN-γ production of both NK cells and NKDC ( Conclusion: IL-18, IL-12, and TLR9 ligation regulate NKDC IFN-γ production in response to LM. This work was supported by grant DK068346. |
| Databáze: | OpenAIRE |
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