Interactions Between Insulin and Angiotensin II on Calcium Mobilization in Skin Fibroblasts from Insulin Resistant Patients with Essential Hypertension

Autor:	Ceolotto Giulio, Baritono Elisabetta, Valente Roberto, Monari Alessandra, Reato Stefania, Sartori Michelangelo, Trevisan Roberto, Semplicini Andrea
Rok vydání:	2000
Předmět:	Internal Medicine
Zdroj:	Hypertension. 36:726-726
ISSN:	1524-4563 0194-911X
DOI:	10.1161/hyp.36.suppl_1.726
Popis:	P181 Objectives: Insulin attenuates angiotensin II (Ang II)-induced Cai2+ mobilization in insulin sensitive (IS) control subjects. Therefore we compared the interactions between Ang II and insulin on Cai2+ mobilization in skin fibroblasts from insulin resistant (IR) and insulin sensitive (IS) patients with essential hypertension (HT). Methods: Skin fibroblasts from 9 normotensives (NT) and 18 HT were cultured and used after 4 passages. Ca2+ was measured in monolayers of 24h serum-deprived cells using Fura-2 AM. mRNA expression of Gαi2 subunit was determined by RT-PCR using a 5 and 3 specific primers. Results: Resting and Ang II-stimulated (100nM) Cai2+ peak were higher in fibroblasts from HT than NT (76±3 vs 62±3 nM, p
Databáze:	OpenAIRE
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