Diffusion coefficients of oligonucleotides in capillary gel electrophoresis

Autor:	Maria Helen Kleemiß, Hong‐Feng Yin, Jürgen A. Lux, Gerhard Schomburg
Rok vydání:	1991
Předmět:	Electrophoresis Chromatography Capillary electrophoresis Gel electrophoresis of nucleic acids Oligonucleotide Capillary action Chemistry Mechanical Engineering Diffusion Analytical chemistry Molecule Filtration and Separation Polyacrylamide gel electrophoresis
Zdroj:	Journal of Microcolumn Separations. 3:331-335
ISSN:	1520-667X 1040-7685
Popis:	Diffusion coefficients of oligonucleotides have been measured in polyacrylamide gel capillaries without application of an electrical field. A relationship between such diffusion coefficients and molecular size of the oligonucleotides could be achieved. The slower diffusion of larger oligonucleotides cannot explain the high separation efficiencies of capillary gel electrophoretic separations alone. By theoretical considerations it became obvious that diffusion coefficients of oligonucleotide molecules and also their intermolecular interaction with the polyacrylamide gel may be considerably changed by application of electrical fields.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d95403f6b8e29af219ac09ed39de9578 https://doi.org/10.1002/mcs.1220030407 Zobrazit plný text záznamu Plný text