Estradiol-17β Triggers Luteinizing Hormone Release in the Protandrous Black Porgy (Acanthopagrus schlegeli Bleeker) Through Multiple Interactions with Gonadotropin-Releasing Hormone Control1
| Autor: | Chun-Lin He, Fan-Pin Yen, Sylvie Dufour, Ching-Fong Chang, Lian-Tien Sun, Yan-Horn Lee, Jing-Duan Huang |
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| Rok vydání: | 2002 |
| Předmět: |
endocrine system
medicine.medical_specialty medicine.drug_class Stimulation Cell Biology General Medicine Gonadotropin-releasing hormone Biology In vitro Basal (phylogenetics) Endocrinology Reproductive Medicine In vivo Internal medicine medicine Gonadotropin Luteinizing hormone hormones hormone substitutes and hormone antagonists Testosterone |
| Zdroj: | Biology of Reproduction. 66:251-257 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod66.1.251 |
| Popis: | We investigated the mechanism of estradiol-17β (E2) action on stimulation of LH (=gonadotropin II) release in the black porgy fish (Acanthopagrus schlegeli Bleeker) using an in vivo approach and primary cultures of dispersed pituitary cells in vitro. In vivo, E2 but not androgens (testosterone [T] and 11-ketotestosterone [11-KT]) significantly stimulated plasma LH in a dose-dependent manner. Estradiol-17β also increased brain content of seabream GnRH. GnRH antagonist prevented E2 stimulation of LH release in vivo, indicating that the effect of E2 on LH was mediated by GnRH. In vitro, sex steroids (E2, T, 11-KT) alone had no effect on basal LH release in the cultured pituitary cells, but GnRH significantly stimulated LH release. Estradiol-17β potentiated GnRH stimulation of LH release, an effect that was inhibited by GnRH antagonist, and 11-KT, but not T, also potentiated GnRH stimulation of LH release. The potentiating effect of 11-KT on GnRH-induced LH release in vitro was stronger than that of ... |
| Databáze: | OpenAIRE |
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