Activation of adipose tissue macrophages in obese mice does not require lymphocytes

Autor: James W. Behan, Xia Sheng, Steven D. Mittelman, Xingchao Wang, Yong-Mi Kim, Ehsan A. Ehsanipour, Rocky Pramanik, Yao-Te Hsieh
Rok vydání: 2013
Předmět:
Zdroj: Obesity. 21:1380-1388
ISSN: 1930-7381
DOI: 10.1002/oby.20159
Popis: Objective Macrophages which infiltrate adipose tissue and secrete proinflammatory cytokines may be responsible for obesity-induced insulin resistance. However, the reason why macrophages migrate into adipose tissue and become activated remains unknown though some studies suggest that this may be regulated by T and B lymphocytes. In this study, it has been tested whether T and B lymphocytes and natural killer (NK) cells were necessary for the obesity-induced activation of macrophages in adipose tissue. Design and Methods NOD/SCID/IL2-receptor gamma-chain knockout (NSG) mice, which lack mature T and B lymphocytes and NK cells, were made obese by selectively reducing litters and weaning onto a high-fat diet. Mice were then maintained on the diet for 10–11 weeks. Results Adipose tissue from obese NSG mice had more activated macrophages than nonobese mice. These macrophages were found in “crown-like structures” surrounding adipocytes, and expressed higher levels of the inflammatory cytokine TNF-α. However, obesity did not impair glucose tolerance in the NSG mice. Conclusions These studies demonstrated that T and B lymphocytes and NK cells are not necessary for adipose tissue macrophage activation in obese mice. T and B lymphocytes and/or NK cells may be necessary for the development of obesity-induced impaired glucose tolerance.
Databáze: OpenAIRE
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