Obesity-driven alterations in adipose-derived stem cells are partially restored by weight loss
| Autor: | Beatriz G. Gálvez, Nuria San Martín, Javier Suárez, Beatriz de Lucas, Aurora Bernal, Laura M. Pérez |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Nutrition and Dietetics Cell growth Endocrinology Diabetes and Metabolism Cellular differentiation Medicine (miscellaneous) Adipose tissue Biology medicine.disease 03 medical and health sciences 030104 developmental biology Endocrinology medicine.anatomical_structure Weight loss Internal medicine Diabetes mellitus Immunology medicine medicine.symptom Stem cell Tissue homeostasis Subcutaneous tissue |
| Zdroj: | Obesity. 24:661-669 |
| ISSN: | 1930-7381 |
| DOI: | 10.1002/oby.21405 |
| Popis: | Objective The therapeutic potential of adipose-derived stem cells (ASCs) is reduced by various stress-inducing conditions that affect tissue homeostasis such as diabetes, aging, and obesity. Previous works have provided evidence of negative effects of obesity on ASC populations, but it is unclear whether this persists after a weight loss. This study evaluated whether weight loss can restore the attenuated properties found in ASCs derived from populations with obesity (oASCs). Methods In vitro functional analyses were performed to investigate the possible recovery properties in mouse oASCs. Using ASCs isolated from subcutaneous tissue from formerly obese mice (dASCs) and control mice (cASCs), cell proliferation, viability, and some regenerative properties in these cells were analyzed compared with oASCs to evaluate the functional cell state. Results Cell proliferation, viability, and some regenerative properties are strengthened in dASCs and cASCs compared with oASCs. Nevertheless, metabolic analysis reveals a mitochondrial load misbalance and function leading to impaired respiration in dASCs. Conclusions This study demonstrates that an initial obese environment triggers a detrimental state in ASCs that is not completely recovered after weight loss. |
| Databáze: | OpenAIRE |
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