Autor: T. N. Lepikhova, Sakharova NIu, Joyce Tombran-Tink, Valery M. Lipkin, Malashenko Am, Kostanian Ia, Konstantin Lepikhov, Navolotskaia Ev
Rok vydání: 2002
Předmět:
Zdroj: Russian Journal of Developmental Biology. 33:158-163
ISSN: 1062-3604
DOI: 10.1023/a:1015674908691
Popis: We studied the effect of synthetic peptides PEDF-6 and HLDF-6 on preimplantation development of mouse embryos in vitro. PEDF-6 peptide corresponds to fragment 351–356 and of pigment epithelium-derived differentiation factor (PEDF), while HLDF-6 peptide corresponds to fragment 84–89 of differentiation factor HLDF isolated from HL-60 cell line. Despite high homology, these peptides had different effects on the early development. PEDF-6 had no effect on the cleavage of 2–4-cell embryos but decelerated blastocyst formation from such embryos and disturbed their structure. In the presence of HLDF-6 the blastomeres divided more actively as compared to the control and a higher number of embryos developed to the blastocyst stage. The effects of both peptides were stage-specific: the affect the embryos at early cleavage stages and, apparently, determine their further development at that moment although do not directly affect formation of the blastocysts.
Databáze: OpenAIRE
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