αvβ3 and αvβ5 Integrin Expression in Glioma Periphery
| Autor: | David A. Cheresh, Demetrios C. Nikas, Jianping Zhang, Maura Francolini, Jon F. Strasser, Paola Marthyn, Roberto Villani, Peter McL. Black, Rona S. Carroll, Lorenzo Bello |
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| Rok vydání: | 2001 |
| Předmět: |
Pathology
medicine.medical_specialty Angiogenesis Integrin Cell migration Biology medicine.disease medicine.disease_cause Fibroblast growth factor Vascular endothelial growth factor chemistry.chemical_compound chemistry Glioma medicine Cancer research biology.protein Immunohistochemistry Surgery Neurology (clinical) Carcinogenesis |
| Zdroj: | Neurosurgery. 49:380-390 |
| ISSN: | 1524-4040 0148-396X |
| DOI: | 10.1097/00006123-200108000-00022 |
| Popis: | OBJECTIVEThis study analyzed the expression of integrins αvβ3 and αvβ5 in glioma tissue and focused on the periphery of high-grade gliomas.METHODSThe analysis was performed with Western blot, immunohistochemistry, and immunofluorescence, by use of two monoclonal antibodies able to recognize the functional integrin heterodimer. The expression of integrin-related ligands and growth factors also was studied. Sections from the tumor periphery were classified as either tumor periphery (light tumor infiltrate or scant visible cells) or peritumor (heavy tumor infiltration).RESULTSOur data on glioma tissues demonstrated that both integrins were expressed in glioma cells and vasculature and their expression correlated with the histological grade. αvβ3 expression was prominent in astrocytic tumors. Both integrins were markers of tumor vasculature, particularly of endothelial proliferation. A high-grade glioma periphery demonstrated a prominent expression of integrin αvβ3. Cells demonstrating αvβ3 positivity were identified as tumor astrocytes and endothelial cells by double imaging. The same cells were surrounded by some αvβ3 ligands and co-localized fibroblast growth factor 2. Matrix metalloproteinase 2 also was found to be co-localized with αvβ3 in the same cells. αvβ3 expression was more relevant in tumor astrocytes. αvβ3 integrin and vascular endothelial growth factor expression increased from the periphery to the tumor center.CONCLUSIONOur data support the role of integrins αvβ3 and αvβ5 in glioma-associated angiogenesis. In addition, they suggest a role for integrin αvβ3 in neoangiogenesis and cell migration in high-grade glioma periphery. |
| Databáze: | OpenAIRE |
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