Next-generation sequencing of the mitochondrial genome of Dolichovespula panda (Hymenoptera: Vespidae) with a phylogenetic analysis of Vespidae
| Autor: | Peng-Yan Chen, Jiang-Li Tan, Qing-Qing Tan, Gong Yajun, Xu-Lei Fan, Wei Shujun |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics Mitochondrial DNA Phylogenetic tree Dolichovespula Biology biology.organism_classification 010603 evolutionary biology 01 natural sciences Stop codon Vespula DNA sequencing 03 medical and health sciences 030104 developmental biology Sister group Insect Science Gene |
| Zdroj: | Journal of Asia-Pacific Entomology. 20:971-976 |
| ISSN: | 1226-8615 |
| DOI: | 10.1016/j.aspen.2017.07.009 |
| Popis: | For the first time the mitochondrial genome of a Dolichovespula species, D. panda Archer (Hymenoptera: Vespidae), was sequenced with a next-generation sequencing approach. The sequenced mitochondrial genome is 17137 bp long and consists of 13 protein-coding, 22 tRNA and two rRNA genes, as well as a partial A + T-rich region. Twenty-two of the genes are encoded on the majority strand and 15 genes on the minority strand. All protein-coding genes start with ATN codons and have a TAA termination codon, except for one with a TA codon. Compared with the putative ancestral arrangement of insects, the D. panda mitochondrial genome shows the shuffling of trnN and trnE, and of trnQ and trnM, the translocation of trnY to upstream of trnI, and of trnL1 to the region between trnS2 and nad1 and a reversal of trnS1. A phylogenetic tree within the Vespidae was reconstructed using the 13 protein-coding mitochondrial genes. This shows a sister group relationship between Dolichovespula and a clade formed by Vespa and Vespula. It also corroborated the position of Eumeninae as sister group of the clade Polistinae + Vespinae. |
| Databáze: | OpenAIRE |
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