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In April 2011, a virus was isolated by single lesion isolation on Chenopodium quinoa leaves from an amaryllis plant with chlorotic ringspots at a private garden in Changhua County, Taiwan. The Illumina MiSeq sequencing system was used to determine the genomic nucleotide (nt) sequence of the virus. A de novo assembled contig with 9377 nt, containing a putative open reading frame of the potyviral polyprotein, was annotated as the potyvirus Amazon lily mosaic virus (ALiMV) by sharing 95.5% nt identity with a partial genomic sequence of ALiMV available in GenBank. Therefore, the amaryllis virus was designated as ALiMV-TW. Through 5´ and 3´ rapid amplification of cDNA ends (RACEs), the complete genome sequence of ALiMV-TW was clarified as 9618 nt. Sequence analysis indicated that the genome and polyprotein of ALiMV-TW share 52.3%-65.1% nt identity and 30.1%-64.2% aa identity with those of other potyviruses, respectively. This is the first report to elucidate the complete genome sequence of Amazon lily mosaic virus. |
