Electrophoretic karyotype of the zygomycete Absidia glauca: evidence for differences between mating types

Autor:	Johannes Wöstemeyer, Torsten Kayser
Rok vydání:	1991
Předmět:	Genetics Elongation factor Transformation (genetics) Mating type Gene mapping Karyotype General Medicine Biology Ribosomal DNA Gene Molecular biology Southern blot
Zdroj:	Current Genetics. 19:279-284
ISSN:	1432-0983 0172-8083
Popis:	Experimental conditions for the separation of chromosomes from the model zygomycete Absidia glauca by rotating field electrophoresis were established. The sexually compatible strains of the mating type pair A. glauca CBS 100.48 (+) and CBS 101.48 (-) showed considerable differences in their electrophoretic karyotype. By Southern hybridization with homologous probes we have mapped the chromosomal locations for rDNA, the repetitive element rAg1, and the genes for actin and the elongation factor EF1. For the mapping of ubiquitin information we used a heterologous probe from U. maydis. The combination of electrophoretic karyotyping and Southern mapping proved to be a useful tool for characterizing mutant genotypes, which were induced by integrative transformation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d8f27e4a067ddebcde379c7d79a64b7f https://doi.org/10.1007/bf00355055 Zobrazit plný text záznamu Full text from SpringerLink