Graphite Furnace Atomic Absorption Spectrophotometric Determination of 4-Hydroxy-3-Nitrobenzenearsonic Acid, Other Organic Arsenicals, and Inorganic Arsenic in Finished Animal Feed
| Autor: | Frahm Lj, George Gm, McDonnell Jp |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Ammonium carbonate
Aqueous solution Chromatography medicine.diagnostic_test Animal feed Inorganic chemistry chemistry.chemical_element General Chemistry law.invention chemistry.chemical_compound chemistry law Spectrophotometry Roxarsone medicine Atomic absorption spectroscopy Graphite furnace atomic absorption Arsenic |
| Zdroj: | Journal of AOAC INTERNATIONAL. 65:711-719 |
| ISSN: | 0004-5756 |
| DOI: | 10.1093/jaoac/65.3.711 |
| Popis: | 4-Hydroxy-3-nitrobenzenearsonic acid (roxarsone) is administered in animal feed as a growth stimulant over a concentration range of 25-50 ppm. The drug is extracted from 5 g feed with 200 mL aqueous 1.0% ammonium carbonate solution and 5 min of mechanical shaking. Undissolved feed particles are allowed to settle and 1.0 mL aliquot of extract is diluted with 9.0 mL 15% methanol solution. This solution is subjected to sample atomization by a graphite furnace and arsenic detection by atomic absorption spectrophotometry (AAS). Roxarsone recovery from nonmedicated commercial feed fortified at 25 ppm was 103.6% with a relative standard deviation (RSD) of 4.0%. Recovery for 50 ppm fortification was 104.5% (RSD 4.3%). Roxarsone assay results by furnace AAS were compared with results by the current AOAC spectrophotometric method and the AOAC total arsenic method. Results by the 3 methods compare well. The procedure was also used to determine other organic arsenicals and inorganic arsenic in laboratory-fortified feed samples; these recoveries were essentially theoretical. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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