Surface Plasmon Resonance/Surface Plasmon Enhanced Fluorescence: An Optical Technique for the Detection of Multicomponent Macromolecular Adsorption at the Solid/Liquid Interface
| Autor: | Joon-H. Kim,†,‡, and Channing R. Robertson, A. J. Poulose, Alice P. Gast, James T. Kellis, Shaunak Roy |
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| Rok vydání: | 2002 |
| Předmět: |
biology
Chemistry education Surface plasmon Analytical chemistry Substrate (chemistry) Surfaces and Interfaces Condensed Matter Physics Fluorescence Adsorption Electrochemistry biology.protein General Materials Science Surface plasmon resonance Spectroscopy Avidin Macromolecule Localized surface plasmon |
| Zdroj: | Langmuir. 18:6319-6323 |
| ISSN: | 1520-5827 0743-7463 |
| DOI: | 10.1021/la025578w |
| Popis: | We describe an optical technique for the measurement of macromolecular adsorption at the solid/liquid interface when multiple species are present. The technique combines surface plasmon resonance (SPR) with simultaneous surface plasmon enhanced fluorescence (SPEF). The relative ease of construction and linear correlation between SPR and SPEF signals make the technique amenable for coadsorption studies or multiple ligand binding experiments. Here, we demonstrate the utility of the technique with a biotin/avidin/BSA “sandwich” experiment. We then apply SPR/SPEF for the simultaneous monitoring of enzyme adsorption and substrate cleavage of a protease interacting with a substrate surface. |
| Databáze: | OpenAIRE |
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