Cellulolytic properties of Chaetomium crispatum
| Autor: | Herman A. M. Geeraerts, Erick Vandamme |
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| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Journal of Chemical Technology and Biotechnology. Biotechnology. 33:107-113 |
| ISSN: | 1935-1828 0264-3421 |
| DOI: | 10.1002/jctb.280330205 |
| Popis: | The cellulolytic properties of a Chaetomium crispatum strain were investigated. The cellulolytic enzyme complex i.e.: exo-1,4-β-glucosidase (EC 3.2.1.74); endo-1,4-β-glucanase (EC 3.2.1.4.) and β-glucosidase or cellobiase (EC 3.2.1.21) displayed optimal activity at pH 5.0 and 25°C. Although carboxymethyl-celluloses are the usual pseudo-substrates for this enzyme complex, those with a high degree of substitution gave rise to poor growth and low cellulase activity. Insoluble crude cellulosics such as newsprint, recycled paper, rice and flax straw were substantially solubilised at 28°C within 3–5 days of fermentation. A study of the cellulase-complex formation during the growth cycle revealed that β-glucosidase was produced mainly intracellularly in the early exponential phase, while the overall exo-1,4-β-glucosidase and endo-1,4-β-glucanase formation gradually increased during the total fermentation cycle. The mycelial protein of Chaetomium crispatum grown on crude cellulosics displayed a favourable amino acid pattern, indicating its potential value as a source of single cell protein (SCP). |
| Databáze: | OpenAIRE |
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