The Method of Calibration Curves for Immunochromatographic Express Tests. Part 1. Immunochromatographic Express Tests with Colloidal Gold

Autor: Yu. V. Kiseleva, Boris B. Dzantiev, Yu. Yu. Vengerov, Anna N. Berlina, Anatoly V. Zherdev, V. M. Malyuchenko, Yu. A. Kudeyarov, Ya. A. Korolenko, Nadezhda A. Taranova, S. S. Golubev
Rok vydání: 2013
Předmět:
Zdroj: Measurement Techniques. 55:1425-1433
ISSN: 1573-8906
0543-1972
DOI: 10.1007/s11018-013-0144-y
Popis: The operating principle of immunochromatographic test systems is considered. It is noted that with the use of such tests an immunoassay is implemented as a sandwich method in the form of a direct dependence of the luminescence of test stripes on the concentration of the test substance or as a competitive method with an inverse dependence. An experimental procedure of obtaining such dependences, which is necessary for the construction of calibration curves, is set forth. Exponential expressions for arriving at an analytic description of the associated curves are proposed; moreover, the coefficients in these expressions are determined by the method of least squares. An analysis of the error of the method of calibration curves that may be used to obtain quantitative results of the analysis is presented.
Databáze: OpenAIRE
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