Micropatterning of Covalently Attached Biotin on Poly(lactic acid) Film Surfaces

Autor:	Rahul M. Rasal, Douglas E. Hirt
Rok vydání:	2009
Předmět:	Streptavidin Polymers and Plastics Chemistry Bioengineering Lactic acid Biomaterials Contact angle chemistry.chemical_compound Biotin Biotinylation Polymer chemistry Materials Chemistry Biotechnology Micropatterning Acrylic acid Carbodiimide
Zdroj:	Macromolecular Bioscience. 9:989-996
ISSN:	1616-5187
DOI:	10.1002/mabi.200800374
Popis:	A two-step approach was used to micropattern covalently attached biotin on the surface of poly(lactic acid) (PLA) film. Poly(acrylic acid) (PAA) was micropatterned on PLA using photolithography in step 1, followed by carbodiimide wet chemistry to covalently attach biotin to acid groups in step 2. The PAA micropatterning reaction progression was monitored using attenuated total reflectance-Fourier transform infrared (ATR-FT-IR) spectroscopy, water contact angle goniometry, and atomic force microscopy (AFM). The PAA-biotin conjugation reaction characterization using XPS confirmed the carbodiimide mediated amidation reaction. The resultant PLA film was then immersed into a solution of fluorescence-conjugated streptavidin and examined under fluorescence microscopy to reveal various micropatterns.
Databáze:	OpenAIRE
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