Evaluation of autophagy induction on HEV 239 vaccine immune response in a mouse model
| Autor: | Mehdi Khateri, Parvaneh Mehrbod, Fatemeh Fotouhi, Arash Arashkia, Hamed Naziri, Ehsan Ollah Jazaeri, Sepideh Shahbazi, Mohammad Reza Aghasadeghi, Asghar Abdoli, Fatemeh Motevalli, Azam Bolhassani |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
viruses Intracellular parasite Clinical Biochemistry Autophagy Cell Biology Biology medicine.disease_cause Vaccine efficacy Acquired immune system Biochemistry Virology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Immune system Hepatitis E virus 030220 oncology & carcinogenesis Humoral immunity Genetics medicine Molecular Biology CD8 |
| Zdroj: | IUBMB Life. 70:207-214 |
| ISSN: | 1521-6543 |
| DOI: | 10.1002/iub.1719 |
| Popis: | Hepatitis E virus (HEV) infection remains a serious threat to life and productivity in developing world. Vaccine seems to be an effective, safe, and affordable approach to address HEV disease burden. The HEV genome consists of three open reading frames (ORFs). Of these, ORF2 encodes a single structural protein (pORF2) for the HEV capsid which has been studied extensively as vaccine candidates. Recently, it has been recognized that autophagy plays an important role in innate and adaptive immunity defense against intracellular pathogens. This mechanism could therefore promote a protective immune response by inducing CD4+ and CD8+ T cells. In this study, HEV 239 and Beclin1 proteins were expressed in prokaryotic host cell [Escherichia coli (BL21)]. HEV 239 protein with different formulations (+Alum, +Beclin1, and +Alum-Beclin1) were used as candidate vaccines and administrated subcutaneously in BALB/c mice on 0, 14, and 28 days. Finally, elicited cellular and humoral immunity were evaluated. Taken together, although our results indicated that mice immunized with HEV 239 protein formulated with Alum, Beclin1, and Alum + Beclin1 displayed humoral and cellular response that was not significant in comparison with each other (P > 0.05); whereas they were significant while compared with control groups (P < 0.05). A comprehensive understanding of the intricate interplay between autophagy and immune response remains to be unraveled. Further study will clear the detailed impact of autophagy manipulation to enhance vaccine efficacy and boost the immune responses against the disease. © 2018 IUBMB Life, 70(3):207-214, 2018. |
| Databáze: | OpenAIRE |
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