| Autor: |
Molly Fahey, Juanita J. Anders, Ronald W. Waynant, Kunal Mitra, Darrell B. Tata |
| Rok vydání: |
2007 |
| Předmět: |
|
| Zdroj: |
SPIE Proceedings. |
| ISSN: |
0277-786X |
| DOI: |
10.1117/12.699120 |
| Popis: |
The role of low light intensity in suppressing metabolic activity of transformed cell lines was investigated through the applications of a 1,552nm wavelength pulsed picosecond laser. Human malignant glioblastoma, human leukemia HL-60, and the NIH 3T3 cell lines were used. The cells were grown in 96 well plates and exposed in their respective growth culture media with 10% (v/v) fetal bovine serum under various fluence exposure conditions ranging from 0.115 - 100 J/cm2. All cell lines were exposed at a constant average intensity value of 0.115 W/cm2; 25 kHz repetition rate with 1.6 micro-joule per pulse; pulse duration = 2.93 picosecond. The human malignant glioblastoma and the HL-60 cell lines exhibited a monotonic decline in metabolic activity (down 50 - 60%) relative to their respective sham exposed control counterparts between the fluence values of 0.115 J/cm2 to 10 J/cm2. The NIH 3T3 cells exhibited a maximum suppression of metabolic activity at the fluence value of 50 J/cm2. Metabolic activity was measured through the colorimetric MTS metabolic assay. Interestingly, for all cell lines the metabolic activity was found to return back to the sham exposed control levels as the fluence of exposure was increased up to 100J/cm2. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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