DNA polymerasesδandein developing and aging rat brain

Autor:	Kalluri Subba Rao, Danam Rebecca Prapurna
Rok vydání:	1997
Předmět:	DNA clamp Developmental Neuroscience biology DNA polymerase DNA polymerase II DNA replication biology.protein Primer (molecular biology) DNA polymerase delta DNA polymerase mu Molecular biology Developmental Biology Proliferating cell nuclear antigen
Zdroj:	International Journal of Developmental Neuroscience. 15:67-73
ISSN:	1873-474X 0736-5748
DOI:	10.1016/s0736-5748(96)00047-0
Popis:	Our study reveals the presence of DNA polymerases δ and e, participating in DNA replication and repair, along with already known polymerases α and β, in the developing and aging rat brain. This was achieved through a protocol that takes advantage of the reported differential sensitivities of different DNA polymerases towards certain inhibitors such as butylphenyl and butylanilino nucleotide analogs, 2′, 3′-dideoxythymidine triphosphate, the monoclonal antibody of human polymerase α and the use of preferred template primers and proliferating cell nuclear antigen. The results indicate that while polymerase β seems to be the predominant one, significant levels of polymerases α, δ and e are also present at all the postnatal ages studied and that the relative proportion of polymerase e increases with age. The data suggest that the rat brain is equipped with a sustained DNA repair capacity throughout the life span.
Databáze:	OpenAIRE
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