Optimization of extraction techniques and quantification of swertiamarin and mangiferin by using RP-UFLC method from eleven Swertia species

Autor:	Vishwas A. Bapat, Nikhil B. Gaikwad, Parthraj R. Kshirsagar, Sandeep R. Pai
Rok vydání:	2017
Předmět:	Chromatography biology 010405 organic chemistry Chemistry 010401 analytical chemistry Extraction (chemistry) Plant Science biology.organism_classification 01 natural sciences Swertia 0104 chemical sciences chemistry.chemical_compound Phytochemical Extraction methods Mangiferin
Zdroj:	South African Journal of Botany. 108:81-89
ISSN:	0254-6299
Popis:	Present work investigated an efficient method for extraction of swertiamarin and mangiferin from different species of genus Swertia . Various extraction methods like static extraction (SE), continuous shaking extraction (CSE), and ultrasonic extraction (USE) were evaluated for increasing recovery percentage of swertiamarin and mangiferin. The quantification was done using reversed phase-ultra flow liquid chromatographic (RP-UFLC) method at 238 nm (swertiamarin) and 257 nm (mangiferin) wavelength. The results revealed that the percentage extraction of swertiamarin and mangiferin from different species of Swertia by SE was more proficient. Three time intervals were optimized by SE and it was observed that the 24 h extraction gave the maximum recovery in S. chirayita of swertiamarin (256.98 mg/g) and mangiferin (155.76 ± 7.78 mg/g). S. minor remarkably best match for S. chirayita as per phytochemical fingerprint using swertiamarin and mangiferin is concerned, suggesting an alternative for chirayita.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d872041b36cfe0134ea49127fc7e79c6 https://doi.org/10.1016/j.sajb.2016.09.016 Zobrazit plný text záznamu Full Text from ScienceDirect