| Autor: |
Stoyl P. Stoylov, Sv. Jovtchev, Hans Bäumler, M. Marinov, I. Dzhenev, S. Stoeff |
| Rok vydání: |
2002 |
| Předmět: |
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| Zdroj: |
Colloids and Surfaces A: Physicochemical and Engineering Aspects. 209:257-265 |
| ISSN: |
0927-7757 |
| DOI: |
10.1016/s0927-7757(02)00188-7 |
| Popis: |
Electric light scattering technique is applied for investigation of red blood cells (RBC) surface electric properties, geometric and mechanical characteristics and interactions with neutral polymers. The electro-optically determined electric polarizability of RBC was found to depend on the surface electric charge. Fine glycocalyx structure alterations were indicated through electro-optical measurements. The diameter of RBC estimated through the electro-optically determined rotational diffusion coefficient is in very good agreement with literature data. RBC glycocalyx-solution interface viscosity, in the presence of dextran 75,000 obtained by electro-optics, is in remarkable agreement with electrophoretically and electrorotationally obtained data found in the literature. All three electrokinetic methods indicated polymer depletion on the RBC surface. Electro-optics, however, was additionally able to detect fine difference in the viscosity profiles at the RBC with altered glycocalyx structure. Electro-optical behavior of RBC was found to depend also on cell deformability. Relative deformability parameter estimated electro-optically was found to be correlated also to cytoplasmic properties—mean cellular hemoglobin. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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