MYCOLOGICAL EXAMINATION OF FISH FEED STUFF WITH SPECIAL REFERENCE TO MYCOTOXIN PRODUCTION
| Autor: | Amany M. Diab, Maha Kholife, Amgad A. Moawad, Abeer El-keredy |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Ochratoxin A
0303 health sciences Veterinary medicine Aspergillus Aflatoxin General Veterinary biology food and beverages Aspergillus flavus 04 agricultural and veterinary sciences biology.organism_classification Commercial fish feed 03 medical and health sciences chemistry.chemical_compound chemistry 040102 fisheries 0401 agriculture forestry and fisheries Aspergillus versicolor Mycotoxin Aspergillus ochraceus 030304 developmental biology |
| Zdroj: | SLOVENIAN VETERINARY RESEARCH. 56 |
| ISSN: | 1580-4003 |
| DOI: | 10.26873/svr-769-2019 |
| Popis: | Mycotoxin contamination is considered as one of the most economic problems for livestock and feed industries. A total of thirty fish feedstuffs were collected for isolation and identification of fungi from fish farms in Kafr El Sheikh Governorate, Egypt. Five fungal genera were isolated from all examined feedstuffs. The most frequent isolated fungi was Aspergillus spp. (86.66%) followed by Penicillium spp. (23.33%), and Fusariam spp. (10%), Mucor spp. (6.66%) then Rhizopus spp. (3.33%). Aspergillus niger was the most prevailing genus (43.33%) followed by Aspergillus flavus ( (30%), and Aspergillus fumigatus (6.66%), Aspergillus versicolor (3.33%) then Aspergillus terrus (3.33%). Aspergillus ochraceus was not found despite the presence of ochratoxin A (OTA) in low concentrations. Some mycotoxins were produced by more than one fungal species. Mycotoxins determination using HPLC revealed that 23.33% (7/30) of examined fish feed samples were positive, while 23 samples (76.66%) were mycotoxins free. In positive samples of mycotoxins, aflatoxins (AF) and OTA represented 23.33%, 10%, respectively. The estimated carcinogenic aflatoxins were AFB 1 , AFB 2 , AFG 1 with a percentage of 23.33%, 13.34%, 6.67%, respectively, but AFG 2 was not found. About 42.86% of the AFs producing fish feeds was higher than the permissible limit of aflatoxins (permissible limit is 20 ppb). The aflatoxigenic ability of the recovered nine A. flavus referred to 6 out of 9 (66.67%) were aflatoxin producers. Polymerase chain reaction (PCR) was carried out using the norsolonic acid reductase ( nor ), versicolorin A ( ver-1 ) and O-methylsterigmatocystin ( omtA ) as aflatoxin producing genes of the isolated A. flavus strains. From the AF producing A. flavus , 50% was above the permissible limit. Therefore, the high contamination of fish feedstuffs with fungi, AFB 1 andlow OTA in fish farms indicated potential risks to fish liveliness, derived products and the health of fish consumers. Key words : fish feedstuff; fungi; aflatoxins; ochratoxin A |
| Databáze: | OpenAIRE |
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